Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

SRP0119

Sigma-Aldrich

Sirtuin 5 human

recombinant, expressed in E. coli, ≥50% (SDS-PAGE)

Synonym(s):

NAD-dependent ADP-ribosyltransferase sirtuin-5, SIR2L5, SIRT5, sir2-like 5

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

human

recombinant

expressed in E. coli

assay

≥50% (SDS-PAGE)

form

aqueous solution

mol wt

59.8 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

NM_012241

UniProt accession no.

Q9NXA8

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SRP5272SRP0120SRP0118
Sirtuin 5 human recombinant, expressed in E. coli, ≥50% (SDS-PAGE)

SRP0119

Sirtuin 5 human

SIRT5, active, His tagged human recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

SRP5272

SIRT5, active, His tagged human

Sirtuin 6 human recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

SRP0120

Sirtuin 6 human

Sirtuin 4 human recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

SRP0118

Sirtuin 4 human

assay

≥50% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

biological source

human

biological source

-

biological source

human

biological source

human

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

form

aqueous solution

form

buffered aqueous glycerol solution

form

aqueous solution

form

aqueous solution

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

packaging

pkg of 100 μg

packaging

-

packaging

pkg of 100 μg

packaging

pkg of 100 μg

General description

The sirtuin 5 (SIRT5) gene with eight exons is mapped to human chromosome 6p23. The gene exists in two isoforms codings for 310 amino acid and 299 amino acid protein, respectively. Sirtuin 5 is mainly present in heart muscle cells and in lymphoblasts. It is a member of the silent information regulator 2 (Sir2) family of sirtuin histone deacetylases (HDACs).[1] Sirtuin 5 is a mitochondrial protein.[2]
Human Sirtuin 5, GenBank Accession No. NM_012241), full length with N-terminal ST tag, MW = 59.8kDa, expressed in Escherichia coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Sirtuin 5 (SIRT5) catalyzes the degradation of negatively charged acylated substrates. It also possesses desuccinylase, demalonylase and deglutarylase activities.[2] SIRT5 is a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase and is overexpressed in non-small cell lung cancer (NSCLC) and may have a role in cancer growth.[1][3] Members of sirtun family play a vital role in epigenetic gene silencing, DNA repair and recombination, cell-cycle, microtubule organization, and in the regulation of aging.[1] SIRT5 has been involved in regulation of the carbamoyl phosphase synthase 1 activity, an essential enzyme of urea cycle, via maintaining lysine glutarylation levels. Sirtuin 5 as a mitochondrial protein plays a major role in controlling ATP production, apoptosis, and cell signaling.[4]

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    50016080903
    5001680903

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Role of the Substrate Specificity-Defining Residues of Human SIRT5 in Modulating the Structural Stability and Inhibitory Features of the Enzyme.
    Yu J
    PLoS ONE, 11(3), e0152467-e0152467 (2016)
    Assignment of the NAD-dependent deacetylase sirtuin 5 gene (SIRT5) to human chromosome band 6p23 by in situ hybridization.
    Mahlknecht U
    Cytogenetic and genome research, 112(3-4), 208-212 (2006)
    Chemical probing of the human sirtuin 5 active site reveals its substrate acyl specificity and peptide-based inhibitors.
    Roessler C
    Angewandte Chemie (International Edition in English), 53(40), 10728-10732 (2014)
    SIRT5 facilitates cancer cell growth and drug resistance in non-small cell lung cancer.
    Lu W
    Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 35(11), 10699-10705 (2014)
    Rommel A Mathias et al.
    Cell, 159(7), 1615-1625 (2014-12-20)
    Sirtuins (SIRTs) are critical enzymes that govern genome regulation, metabolism, and aging. Despite conserved deacetylase domains, mitochondrial SIRT4 and SIRT5 have little to no deacetylase activity, and a robust catalytic activity for SIRT4 has been elusive. Here, we establish SIRT4
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service