SRP2008

TFIIF (RAP30 subunit) human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Synonym(s): BTF4, RAP30, TF2F2, TFIiF
• UNSPSC Code: 12352202
• NACRES: NA.26

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥80% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~28.4 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 600 μg/mL
• technique(s): western blot: suitable
• color: clear colorless
• NCBI accession no.: NM_004128
• UniProt accession no.: P13984
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... GTF2F2(2963)

Description

Biochem/physiol Actions

The transcription factor IIF (TFIIF) is composed of 58 kDa (RAP74) and 26 kDa (RAP30) subunits that form a heterodimer, and was first identified through the ability to interact with immobilized RNA polymerase II. The RAP30 subunit of TFIIF contains two distinct regions with sequence similarity to E. coli factors and can deliver RNA polymerase II to the promoter to support transcription initiation in the absence of RAP74.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable