SRP3269
RANTES human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonym(s):
CCL5, SIS-delta
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Pricing and availability is not currently available.
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biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
1.0-10.0 ng/mL
mol wt
7.8 kDa
packaging
pkg of 20 μg
impurities
<0.1 EU/μg endotoxin, tested
color
white
UniProt accession no.
1 of 4
This Item | SCC116 | SCC163 | SCC187 |
|---|---|---|---|
| biological source mouse | biological source mouse | biological source human | biological source mouse |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable, cell culture | mammalian: suitable | technique(s) cell based assay: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| shipped in ambient | shipped in ambient | shipped in - | shipped in ambient |
| Quality Level 100 | Quality Level 100 | Quality Level - | Quality Level - |
General description
Regulated on activation, normal T cell expressed and secreted (RANTES) also known as C-C motif chemokine ligand 5 (CCL5) is encoded by the gene mapped to human chromosome 17.[1] RANTES belongs to intercrine or chemokine family of proinflammatory basic polypeptides and is expressed by various hematopoietic and non-hematopoietic cell types involved in homing and migration of effector and memory T cells during acute infections.[2][3] Recombinant human RANTES is a 7.8kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.
Application
RANTES human has been used in following studies:
- In transmigration experiments to determine whether LFA-1 cross-linking could affect peripheral blood T lymphocytes (PBTL) migration in response to the chemokine RANTES.[4]
- Luminol-dependent chemiluminescence assay to determine the effect of RANTES on reactive oxygen species (ROS) production by eosinophils.[5]
Biochem/physiol Actions
Regulated on activation, normal T cell expressed and secreted (RANTES) is a chemoattractant towards monocytes, dendritic cells (DCs), memory T cells (CD4+/CD45RO), basophils, and eosinophils.[3] The encoded chemokine protein functions as a ligand for CCR1, CCR3, and CCR5.1 RANTES inhibits the action of R5 strains of HIV-1 via blocking CCR5 receptor. The In1.1C variant of RANTES gene is linked with susceptibility to HIV-1 infection and disease progression. Hence, this can be used as a potent biomarker to determine the prognosis of HIV-1 infection.[1][6]
Physical form
Lyophilized with no additives.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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J J Oppenheim et al.
Annual review of immunology, 9, 617-648 (1991-01-01)
A family consisting of at least ten distinct novel 8-10 kd cytokines has been identified over the past 12 years. These cytokines exhibit from 20 to 45% homology in amino acid sequence, are probably all basic heparin-binding polypeptides, and have
Upregulated response to chemokines in oxidative metabolism of eosinophils in asthma and allergic rhinitis.
Sannohe S
The European Respiratory Journal, 21(6), 925-931 (2003)
RANTES-activated human T lymphocytes. A role for phosphoinositide 3-kinase.
Turner L
Journal of Immunology, 155(5), 2437-2444 (1995)
Role of chemokine and cytokine polymorphisms in the progression of HIV-1 disease.
Mahajan SD
Biochemical and Biophysical Research Communications, 396(2), 348-352 (2010)
A role for the chemokine RANTES in regulating CD8 T cell responses during chronic viral infection.
Crawford A
PLoS Pathogens, 7(7), e1002098-e1002098 (2011)
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