SRP3301

MIDKINE mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(s): MK, NEGF-2
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: 13.4 kDa
• packaging: pkg of 20 μg
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: P12025
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... MDK(17242)

Description

General description

MK plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development. MK chemoattracts embryonic neurons, neutrophils and macrophages, and by signaling through the ALK receptor it exerts angiogenic, growth and survival activities during tumorgenesis.

Biochem/physiol Actions

Midkine and its functionally-related protein Pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK). Recombinant murine Midkine is a 13.4 kDa protein containing 123 amino acid residues including five intra-molecular disulfide bonds.

Physical form

Lyophilized from 0.4x PBS, pH 7.4.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable