SRP3327

IL-1 RECEPTOR ANTAGONIST human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(s): ICIL-1RA, IL-1RN, IL-1Ra, IRAP
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 20-40 ng/mL
• mol wt: 17.2 kDa
• packaging: pkg of 100 μg
• storage condition: (Keep container tightly closed in a dry and well-ventilated place)
• technique(s): activity assay: suitable
• impurities: endotoxin, tested

Description

General description

Research area: Immunology & cytokines

Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring inflammatory inhibitor protein.[1] IL-1Ra is produced by corneal epithelial cells, monocytes, neutrophils, macrophages, and fibroblasts.[2] Recombinant human IL-1Ra is a 17.2kDa protein consisting of 153 amino acid residues.

Application

Interleukin-1 receptor antagonist (IL-1RA) human has been used:

• to study the role of interleukin (IL)-1β receptor in cell senescence induced by doxorubicin[3]
• to block the IL-1β binding to its receptor[4]
• to understand the role of autocrine interleukin-1β (IL-1β) signaling downstream of cl- in RPS27 modulation[5][5][5]

Biochem/physiol Actions

Interleukin-1 receptor antagonist (IL-1RA) inhibits the activity of IL-1α and IL-1β by competitively blocking binding to their type I and type II receptors.[6] Therapeutically, IL-1RA may help in the treatment of sepsis, cachexia, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease.[7][8][9][10][11]

Physical form

Lyophilized from 20 mM Tris, pH 8.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable