SRP5203
LC20, GST tagged from mouse
recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
MGC3505, MLC2, MRLC1, MYL9, MYRL2
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About This Item
biological source
mouse
recombinant
expressed in E. coli
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
~45 kDa
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
mouse ... Myl9(98932)
General description
LC20 is the myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. LC20 protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for LC20 and the deduced 172-amino acid protein is highly conserved, with only 3 differences between the human and chicken proteins. Light chain phosphorylation causes the folded monomeric form of myosin to extend and assemble into filaments. This observation established the involvement of the LC20 in conformational transitions of smooth muscle myosin.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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P D Wagner et al.
The Journal of biological chemistry, 261(17), 7778-7783 (1986-06-15)
Phosphorylation of the 20,000-Da light chains, LC20, of vertebrate smooth muscle myosins is thought to be the primary mechanism for regulating the actin-activated ATPase activities of these myosins and consequently smooth muscle contraction. While actin stimulates the MgATPase activities of
K M Trybus et al.
The Journal of biological chemistry, 263(31), 16485-16492 (1988-11-05)
Light chain phosphorylation causes the folded monomeric form of myosin to extend and assemble into filaments. This observation established the involvement of the 20-kDa regulatory light chain (LC20) in conformational transitions of smooth muscle myosin. To further assess the role
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