T2815

Transforming Growth Factor-β2 human

≥97% (electrophoresis), recombinant, expressed in NSO cells, powder, suitable for cell culture

• Synonym(s): hTGF-β2, TGF-β2
• MDL number: MFCD00163957
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Transforming Growth Factor-β2 human, TGF-β2, recombinant, expressed in NSO cells, powder, suitable for cell culture
• biological source: human
• Quality Level: 200
• recombinant: expressed in NSO cells
• assay: ≥97% (electrophoresis)
• form: powder
• potency: 0.04 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: 25 kDa
• packaging: pkg of 10 μg; pkg of 2 μg

Description

General description

The transforming growth factor-β2 (TGFB2) gene is mapped to human chromosome 1q41.[1] The encoded protein is localized to extracellular matrix.[2] TGF-β2 is a member of the TGF-β superfamily.[3] TGF-β exists in three isoforms (TGF-β1, TGF-β2 and TGF-β3) and are known to share similar structure and function.[4]

Application

Transforming Growth Factor-β2 human has been used in chondrogenic, adipogenic and osteogenic differentiation of mesenchymal stem cells.[5] It has also been used to induce extracellular matrix accumulation in primary human trabecular meshwork cells and further to study the action of tetramethylpyrazine on the same.[6]

Biochem/physiol Actions

TGF-β2 is known to stimulate cell growth, differentiation, migration and survival.[7] TGF-β2 acts as a key regulator for immune homeostasis by controlling lymphocyte proliferation, embryogenesis, hematopoiesis and apoptosis.[6] TGF-β is also associated with inflammation and wound healing and is considered as a strong growth inhibitor for keratinocyte cells.[8][9] TGF-β is associated with the process of tumor development, progression and metastasis.[10] TGF-β2 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis.[7] The TGF-β gene is overexpressed in glioma tumors and also in colon cancer, gastric cancer and cervical lesions.[4]

Transforming growth factor-β2 (TGF-β2), like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.1 mg bovine serum albumin

Analysis Note

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)