T5168
Anti-TUBA4A (TUBA1) Antibody
mouse monoclonal, B-5-1-2
Synonym(s):
Alpha Tubulin Antibody Sigma, Anti Tubulin Sigma, Tubulin Antibody Sigma, Tubulin Antibody Sigma - Monoclonal Anti-α-Tubulin antibody produced in mouse
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Product Name
Monoclonal Anti-α-Tubulin antibody produced in mouse, clone B-5-1-2, ascites fluid
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
B-5-1-2, monoclonal
mol wt
antigen ~50 kDa
contains
15 mM sodium azide
species reactivity
mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat
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This Item | 8.52043 | 8.52049 | 8.52074 |
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| assay ≥90.0% (acidimetric), ≥98.0% (HPLC), ≥98% (TLC) | assay ≥96.0% (HPLC), ≥96.0% (acidimetric), ≥98% (TLC) | assay ≥95.0% (acidimetric), ≥98% (TLC), ≥99.0% (HPLC) | assay ≥94.0% (acidimetric), ≥97.0% (HPLC), ≥98% (TLC), ≥98% (perchloric acid titration) |
| functional group Fmoc | functional group Fmoc | functional group Fmoc | functional group Boc, amine |
| product line Novabiochem® | product line Novabiochem® | product line Novabiochem® | product line Novabiochem® |
| storage temp. 2-30°C | storage temp. 2-30°C | storage temp. 2-30°C | storage temp. 2-30°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the B-5-1-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin is the major building block of microtubules.
The TUBA4A (tubulin α 4a), is mapped to human chromosome 2q35. The gene codes for a member of the a -tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. The α-subunit of tubulin has molecular weight of 50,000.
Specificity
Monoclonal Anti-alpha-Tubulin antibody recognizes an epitope at the C-terminal end of the α-tubulin isoform in various organisms.
Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms.
Immunogen
Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.
Application
Monoclonal Anti-α-Tubulin antibody produced in mouse has been used:
- In immunofluorescence Analysis
- In western blotting/ Immunoblotting
- For immunolabelling cells in electron microscopy
Biochem/physiol Actions
Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).
α-Tubulin is a key regulator of cytoskeletal proteins. It mediates cellular developmental stages such as proliferation, migration, signalling and also maintains the shape of the cell. α-Tubulin controls trafficking, signaling and cellular tensegrity mediated by microtubules. The encoded protein is associated with the development and progression of cancer. α-Tubulin acetylation potentiates the metastatic property of breast cancer. Mutation in TUBA4A is associated with the development of various types of cancers, such as oral cancer, breast cancer, rectal cancer, lung cancer and prostate cancer. In addition, variation in the TUBA4A leads to sporadic amyotrophic lateral sclerosis (ALS).
Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and the cytoskeleton.
α-Tubulin is a key regulator of cytoskeletal proteins. It mediates cellular developmental stages such as proliferation, migration, signalling and also maintains the shape of the cell. α-Tubulin controls trafficking, signaling and cellular tensegrity mediated by microtubules. The encoded protein is associated with the development and progression of cancer. α-Tubulin acetylation potentiates the metastatic property of breast cancer. Mutation in TUBA4A is associated with the development of various types of cancers, such as oral cancer, breast cancer, rectal cancer, lung cancer and prostate cancer. In addition, variation in the TUBA4A leads to sporadic amyotrophic lateral sclerosis (ALS).
Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and the cytoskeleton.
Tubulin α mediates the formation of cytoskeletal protein polymers. It is necessary for cell cycle and controls cellular movement and signaling. Tubulin α is known to maintain the shape of the cells. It is an active part of oncogenesis and progression of different types of cancer. Posttranslationally modified Tubulin α governs microtubule functions including cell trafficking and cellular tensegrity.
Physical form
Liquid
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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