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Key Documents

WH0200734M1

Sigma-Aldrich

Monoclonal Anti-SPRED2 antibody produced in mouse

clone 6G8, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-FLJ21897, Anti-FLJ31917, Anti-Spred2, Anti-sprouty-related, EVH1 domain containing 2

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About This Item

MDL number:
MFCD07784893
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6G8, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

indirect ELISA: suitable
western blot: 1-5 μg/mL

isotype

IgG2aκ

GenBank accession no.

NM_181784

UniProt accession no.

Q7Z698

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SPRED2(200734)

General description

SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM

Immunogen

SPRED2 (NP_861449, 120 a.a. ~ 219 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
EDLIEGSTTSSSTIHNEAELGDDDVFTTATDSSSNSSQKREQPTRTISSPTSCEHRRIYTLGHLHDSYPTDHYHLDQPMPRPCRQVSFPDDDEEIVRINP

Biochem/physiol Actions

Sprouty related EVH1 domain containing 2 (SPRED2) modulates erythroid differentiation in chronic myelogenous leukemia cells. It functions as a negative regulator of the mitogen-activated protein kinase (MAPK) pathway and hematopoiesis in the aorta-gonad-mesonephros region. SPRED2 is downregulated in prostate cancer.

Physical form

Solution in phosphate buffered saline, pH 7.4

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
06069-6G8

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Eli A Stahl et al.
Nature genetics, 42(6), 508-514 (2010-05-11)
To identify new genetic risk factors for rheumatoid arthritis, we conducted a genome-wide association study meta-analysis of 5,539 autoantibody-positive individuals with rheumatoid arthritis (cases) and 20,169 controls of European descent, followed by replication in an independent set of 6,768 rheumatoid
N Kachroo et al.
British journal of cancer, 108(3), 597-601 (2012-11-22)
SPRED1 and 2 are key negative regulators of MAPK signalling in mammalian cells. Here, we investigate the expression and functional role of SPREDs in prostate cancer. A transcriptome bank of microdissected grade-specific primary cancers was constructed and interrogated for transcript
Peter Lock et al.
Biochemical and biophysical research communications, 351(4), 1018-1023 (2006-11-11)
Spred proteins modulate growth factor receptor signaling by inhibiting the Ras-MAPK cascade. Here, we show that Spred-1, Spred-2, and Spred-3 are ubiquitinated in HEK293T cells stimulated with epidermal growth factor (EGF) or pervanadate. Spred-2 tyrosines Y228 and/or Y231 in the

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