ZRB1004
Anti-Stat3 Antibody, clone 4N11, ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Acute-phase response factor, Signal transducer and activator of transcription 3
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CA$480.00
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1 G
CA$480.00
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
4N11, recombinant monoclonal
monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 88.07 kDa
observed mol wt ~88 kDa
1 of 4
This Item | 1.09441 | 1.09439 | 1.09435 |
|---|---|---|---|
| format mixture | format mixture | format mixture | format mixture |
| product line Certipur® | product line Certipur® | product line Certipur® | product line Certipur® |
| grade certified reference material | grade certified reference material | grade certified reference material | grade certified reference material |
| application(s) environmental | application(s) environmental | application(s) environmental | application(s) environmental |
| pH 1.0 (25 °C in H2O) | pH 1.0 (25 °C in H2O) | pH 7.0 (20 °C in H2O) | pH 4.0 (20 °C in H2O) |
| density 1.00 g/cm3 at 20 °C | density 1.00 g/cm3 at 20 °C | density 1.01 g/cm3 at 20 °C | density 1.01 g/cm3 at 20 °C |
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 4N11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Stat3. It targets an epitope with in 11 amino acids from the C-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 11 amino acids from the C-terminal region of human Signal transducer and activator of transcription 3 (Stat3).
Application
Anti-Stat3, clone 4N11 ZooMAb, Cat. No. ZRB1004, is a recombinant Rabbit Monoclonal Antibody that detects Stat3 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Stat3 in SK-N-MC and NIH3T3 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Stat3 in human colon, human liver and human skin tissue sections.
Immunocytochemistry Analysis: A 1:150 dilution from a representative lot detected Stat3 in HUVEC, HeLa, A431, and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Stat3 in human colon, human liver and human skin tissue sections.
Immunocytochemistry Analysis: A 1:150 dilution from a representative lot detected Stat3 in HUVEC, HeLa, A431, and NIH 3T3 cells.
Target description
Signal transducer and activator of transcription 3 (UniProt: P40763; also known as Stat3, Acute-phase response factor) is encoded by the STAT3 (also known as APRF) gene (Gene ID: 6774) in human. STAT3 is expressed in most cell types and is activated by multiple cytokines and growth factors. It is a latent cytoplasmic transcription factor that has a dual function of signal transduction and activation of transcription. It mediates cellular responses to interleukins and growth factors. STAT3 shuttles between the nucleus and the cytoplasm. STAT3 is activated by phosphorylation at Tyrosine 705, which induces dimerization, nuclear translocation and DNA binding. A motif comprising of amino acids 150-162 is essential for its nuclear import. In the nucleus it recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target genes. It binds to DNA as either a homodimer or as a heterodimer with STAT1. Constitutively activated STAT3 is reported in a number of human cancers. STAT3 regulates inflammatory response by regulating differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells. STAT3 may also play an apoptotic role by transactivating BIRC5 expression under LEP activation. Ad cytoplasmic STAT3 is shown to repress macroautophagy by inhibiting EIF2AK2/PKR activity. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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