ZRB1163

Anti-TCF25 Antibody, clone 1B14 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Transcription factor 25;TCF-25;Nuclear localized protein 1;NULP1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1B14, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 76.67 kDa; observed mol wt ~76 kDa
• purified by: using Protein A
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): ELISA: suitable; affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_055787.1
• UniProt accession no.: Q9BQ70
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... TCF25(22980)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B14 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Transcription Factor 25 (TCF25). It targets an epitope within 24 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal half of human Transcription factor 25 (TCF25).

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TCF25 in HeLa cell lysate.

Tested applications

Western Blotting Analysis: A representative lot detected TCF25 in lysates from HEK293 cells (1:10,000 dilution) and A431cells (1:1,000 dilution) and recombinant Human TCF25 (1:1,000 dilution).

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TCF25 in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TCF25 in HeLa and NIH3T3 cells.

Enzyme Immunoassay Analysis (ELISA): A serial of dilutions of this antibody detected full-length, recombinant Human TCF25.

Affinity Binding Assay: A representative lot of this antibody bound full-length, recombinant Human TCF25 protein with a KD of 2.2 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TCF25 in HeLa cell lysate.

Target description

Transcription factor 25 (UniProt: Q9BQ70; also known as TCF-25, Nuclear localized protein 1) is encoded by the TCF25 (also known as KIAA1049, NULP1) gene (Gene ID: 22980) in human. TCF25 is a member of the basic helix-loop-helix (bHLH) family of transcription factors that are important in embryonic development. It is mainly localized in the nucleus but may also be present in cytoplasm to some extent. In the embryo, its highest levels are expressed in the brain and in the adult, highest expression is observed in the heart. It acts as a transcriptional repressor and is reported to repress transcription of serum response factor (SRF) in vitro and may play a role in cardiac development. Its expression is consistently reduced in the failing hearts of patients and in hypertrophic mouse hearts. Overexpression of TCF25 is shown to induce cell death in osteosarcoma Saos2 cells with DNA fragmentation. It renders cancer cells more prone to cell death following treatment with chemotherapeutic drugs, such as staurosporine. It is reported to bind to XIAP and this interaction increases apoptotic cell death. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhang, X., et al. (2020). J. Am. Heart Assoc. 9(16); e016419; Steen, H., and Lindholm, D. (2008). Biochem. Biophys. Res. Commun. 366(2); 432-437).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable