ZRB1206

Anti-CaM Kinase IIα Antibody, clone 1C20 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1C20, monoclonal; recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt ~54.09 kDa; observed mol wt ~54 kDa

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1C20 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects CaM Kinase IIa. It targets an epitope within 19 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human CaM Kinase IIa.

Application

Quality Control Testing

Evaluated by Western Blotting in Rat Brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CaM Kinase IIa in Rat brain tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CaM Kinase IIa in Human Brain tissue lysate.

Affinity Binding Assay:: A representative lot of this antibody bound CaM Kinase IIa peptide with a KD of 2.2 x 10-7 in an affinity binding assay.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected CaM Kinase IIa in human cerebral cortex tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CaM Kinase IIa in human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CaM Kinase IIa, clone 1C20 ZooMAb^®, Cat. No. ZRB1206, is a recombinant Rabbit monoclonal antibody that specifically targets CaM Kinase IIa and is tested in Affinity Binding Assay, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Target description

Calcium/calmodulin-dependent protein kinase type IIa (UniProt: Q9UQM7; also known as EC:2.7.11.17, CaM kinase II subunit alpha, CaMK-II subunit alpha) is encoded by the CAMK2A (also known as CAMKA, KIAA0968) gene (Gene ID: 815) in human. CaM kinase II is one of the most widely studied multi-functional enzyme. It is found in high concentrations in the brain and in some regions, it may constitute up to 2% of the total protein content. CaM kinase II is a complex of about 12 subunits, which exist in four differentially expressed forms (a, b, g, d). The a and b isoforms are most abundant in neurons, whereas d and g isoforms are expressed in most tissues. CaM Kinase II is activated by Ca2+/calmodulin. Binding of calmodulin to calmodulin binding domain (aa 290-300) results in conformational change that relieves intrasteric autoinhibition and allows autophosphorylation of Thr-286 which turns the kinase in a constitutively active form and confers to the kinase a Ca2+-independent activity. CaM Kinase II is shown to be involved in synaptic plasticity, neurotransmitter release, long-term potentiation, and migration of developing neurons. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable