ZRB2632

Anti-Interferon α1 Antibody, clone 1M22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): IFL, IFN-AlphaD, IFN-alpha-1/13, IFNA13, Interferon alpha-1/13, Interferon alpha-D (LeIF D)
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1M22, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1M22 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Interferon 1. It targets an epitope within 18 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal half of human Interferon 1.

Application

Quality Control Testing

Evaluated by Western Blotting with human recombinant Interferon 1 protein fragment.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected human recombinant Interferon 1 protein fragment.

Tested Applications

Enzyme Immunoassay (ELISA): A representative lot of this antibody detected Interferon 1 in ELISA application.

Affinity Binding Assay: The binding affinity of this antibody to Interferon 1 peptide was at least ten thousand-fold (10,000X) higher than that obtained with the non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Interferon alpha-1/13 (UniProt: P01562; also known as Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D (LeIF D)) is encoded by the IFNA1 (also known as IFNA13) gene (Gene ID: 3439) in human. Interferon 1 is a type I interferon. This cytokine is involved in a wide range of immunomodulatory functions, including antiviral, antiproliferative, and immunomodulatory activities. Interferon 1 is primarily expressed by leukocytes, particularly monocytes and macrophages, in response to viral infections, double-stranded RNA, or Toll-like receptor ligands. Upon secretion, it binds to its receptor IFNAR1 and IFNAR2, leading to the activation of various signaling pathways and transcription factors. This induces the expression of numerous interferon-stimulated genes involved in antiviral defense and immunomodulation. Dysregulation of interferon 1 has been implicated in infectious diseases, cancers, and in the pathogenesis of various autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and multiple sclerosis. It a significant target for therapeutic interventions. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: López de Padilla CM, and Niewold, TB, (2016) Gene, 576(1 0 1): 14 21)

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable