ZRB2897

Anti-ATG3 Antibody, clone 3M21 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): APG3L, Autophagy-related protein 3 (APG3-like, EC:2.3.2.-, Protein PC3-96, Ubiquitin-like-conjugating enzyme ATG3, hApg3)
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3M21, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 35.9 kDa; observed mol wt ~38 kDa
• purified by: using Protein A
• species reactivity: human, mouse
• species reactivity (predicted by homology): monkey, rat
• packaging: antibody small pack of 25
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_071933
• UniProt accession no.: Q9NT62
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... ATG3(64422)

Description

General description

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3M21 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects ATG3. It targets an epitope within 18 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human ATG3.

Application

Quality Control Testing

Evaluated by Western Blotting in HEK293T cell lysate.

Western Blotting Analysis: A 1: 1,000 dilution of this antibody detected ATG3 in HEK293T cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ATG3 in NIH/3T3 cell lysate.

Flow Cytometry Analysis: 1 µg from a representative lot detected ATG3 in one million K-562 cells.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected ATG3 in HeLa cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ATG3 in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Ubiquitin-like-conjugating enzyme ATG3 (UniProt: Q9NT62; EC:2.3.2.-; also known as Autophagy-related protein 3 (APG3-like; hApg3), Protein PC3-96) is encoded by the ATG3 (also known as APG3, APG3L) gene (Gene ID: 64422) in human. ATG3 is characterized by a conserved structure typical of E2 ubiquitin-conjugating enzymes, which includes a catalytic cysteine residue essential for its enzymatic activity. ATG3 functions primarily as an E2-like enzyme in the autophagy pathway. It is responsible for the conjugation of ATG8 (LC3 in mammals) to phosphatidylethanolamine (PE) on autophagic membranes. This lipidation process is crucial for the expansion and closure of the autophagosome, a key step in autophagy. ATG3 works in conjunction with ATG7, an E1-like enzyme, to transfer ATG8 to the membrane. Mutations or dysregulation of ATG3 can contribute to various diseases, including neurodegenerative diseases, cancer, and infectious diseases. In cancer altered autophagy can affect tumor progression and response to therapy. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.