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Supelco

Ascentis® C18 (3 µm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

Synonym(s):

Ascentis RP18 HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Pricing and availability is not currently available.

Product Name

Ascentis® C18 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 2.1 mm

material

stainless steel column

Quality Level

100

agency

suitable for USP L1 (Similar to Phenomenex Luna C18)

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

extent of labeling

25% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

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LevGo Inc 20303

packaging

pack of 150 ea (Internal packs of 150), case of 3600 ea

packaging

pack of 500 ea

packaging

pack of 500 ea

packaging

case of 2000 ea (Internal packs of 250), pack of 250 ea

diam.

3.5 in.

diam.

-

diam.

-

diam.

-

dish diam.

89 mm

dish diam.

-

dish diam.

-

dish diam.

-

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Application


  • A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS.: This paper compares methodologies involving the Ascentis® C18 HPLC Column for analyzing ginsenosides, showcasing the column′s utility in herbal medicine research (Hsu et al., 2022).

  • Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Encapsulation Efficiency and In Vitro Release.: Demonstrates the use of the Ascentis® C18 HPLC Column in developing a validated HPLC-UV method for analyzing complex drug delivery systems, highlighting the column′s versatility (Brugnera et al., 2022).

  • Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method.: Utilizes the Ascentis® C18 HPLC Column to ensure the stability and quality of veterinary pharmaceuticals, underlining the column′s application in regulatory compliance (Padivitage et al., 2022).

Features and Benefits

  • Excellent retention
  • Symmetric peak shape
  • High reproducibility
  • Complete LC-MS compatibility

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    281263-06
    281263-05
    281263-04
    281263-03
    281263-02

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    Dipanjan Goswami et al.
    Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
    A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
    Federica Pellati et al.
    Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
    In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
    Federica Vacondio et al.
    Journal of pharmaceutical and biomedical analysis, 46(1), 200-205 (2007-10-26)
    A rapid, simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of the imidazole H(3) antagonist ROS203 in rat plasma, using the superior homologue ROS287 as internal standard. Analyses were performed on an Agilent
    Federica Pellati et al.
    Journal of chromatography. A, 1242, 43-58 (2012-05-09)
    In this study, a detailed phytochemical characterization of Echinacea pallida (Nutt.) Nutt. root extracts and dietary supplements was carried out for the first time by developing advanced chromatographic techniques, based on HPLC with diode array (DAD) and electrospray ionization-mass spectrometry
    Meiyun Shi et al.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 951-952, 129-134 (2014-02-22)
    Aralia mandshrica is a well-known traditional Chinese medicine from Northeast China commonly used to treat digestive, circulatory and immune system disorders. Calenduloside E is one of its bioactive components currently under evaluation as a pure drug. In this study, a
    View All Related Papers

    Articles

    Extraction of Nitroimidazoles from Milk and Eggs using Molecularly Imprinted Polymers

    In this report, SupelMIP SPE – Nitroimidazoles was evaluated for the extraction of nitroimidazoles and metabolites from milk and egg.

    Extractables Study with LC-UV/MS

    Reliable identification and quantification of unknown extractables of a single-use system (SUS) with a 21 component CRM mix for liquid chromatography (LC-UV/MS) methods using Ascentis® C18 column.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service