HPLC Solvents

Non-Polar Solvents in HPLC

Non-polar solvents are widely used in both normal-phase HPLC and reversed-phase HPLC, making them versatile choices for various separation applications. These solvents such as hexane and cyclohexane, are particularly effective for separating non-polar compounds, including hydrocarbons and lipids.

Moderately Polar Solvents in HPLC

Moderately polar solvents can be used in both normal-phase and reversed-phase HPLC, making them suitable for separating a wide range of analytes. They are particularly effective for moderately polar compounds. Ethyl acetate, for example, is commonly used for extracting compounds from biological samples.

Polar Solvents in HPLC

Polar solvents are primarily used in reversed-phase HPLC, where the stationary phase is non-polar, and the mobile phase is polar. Common choices like methanol and acetonitrile are highly effective for separating polar compounds, including amino acids, sugars, and pharmaceuticals.

High Polar Solvents in HPLC

Water is a highly polar solvent commonly used in reversed-phase HPLC, often in combination with other solvents. It serves as a primary component in mobile phases, particularly for analyzing high polar compounds or when gradient elution is employed for improved separation.

HPLC grade Solvents

Our HPLC grade solvents are available under two premium brands: LiChrosolv^® solvents and OmniSolv^® solvents (only in the US and Canada). The Supelco^® portfolio is designed for regulated/accredited application fields, while SigmaAldrich^® portfolio caters to research applications.

HPLC Isocratic Solvents

In isocratic HPLC, the mobile phase composition remains constant throughout the entire separation process. These solvents are microfiltered at 0.2 μm, ensuring stable flow, minimizing the risk of clogging, and enhancing the overall efficiency of the HPLC system. They have a low UV profile, reducing background interference in UV detection and enabling clearer, more accurate analyte measurements. Additionally, these solvents are suitable for LC-UV applications.

HPLC Gradient Solvents

Gradient elution in HPLC involves varying the mobile phase composition during the chromatographic run, making it ideal for separating mixtures with a wide range of retention factors. These solvents are microfiltered at 0.2 μm, exhibit minimal UV absorbance to reduce background interference during detection, and contribute to clearer, more accurate analyte detection. They also support low impurity gradient liquid chromatography (LC) baseline, ensuring high-quality separations.

HPLC Plus Solvents

HPLC Plus solvents exhibit high purity, slow gradient baseline drift, and minimal impurities, making them ideal for demanding analytical applications. Their low UV profile reduces background interference, ensuring clear and precise detection. These solvents are also suitable for gas chromatography (GC) , LC-UV, and LC-MS applications.

UHPLC-MS Solvents

Ultra-high performance liquid chromatography (UHPLC) is a type of liquid chromatography that operates at a higher pressure than traditional HPLC. When coupled with mass spectrometry (MS), it enables precise identification and quantification of molecules based on their mass-to-charge ratio. UHPLC allows for the separation of complex mixtures with greater resolution and speed, enabling the analysis of smaller sample sizes while enhancing sensitivity and precision.

These solvents exhibit an extremely low baseline with minimal impurity peaks, such as phthalates and polyethylene glycol (PEG), which can interfere with the detection and quantification of target analytes. Additionally, they form minimal mass spectrometry adducts, improving the accuracy and reliability of the analysis and demonstrating excellent lot-to-lot reproducibility for consistent performance across different batches.