HPLC Analysis of Fusion Protein on Zenix^®-C SEC-300 vs. Zenix^® SEC-300

column

Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777033)

column

Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777092)

mobile phase

150 mM sodium phosphate buffer, pH 7.0 + 200 mM NaCl

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 214 nm

injection

10 μL

sample

1 mg/mL fusion protein, MW 170 kD, pl 6.8-7.0