HPLC Analysis of Phenols on SUPELCOSIL^™ LC-8

column

SUPELCOSIL LC-8, 15 cm × 4.6 mm I.D., 5 μm particles (58220-U)

mobile phase

[A] 1% acetic acid/methanol: [B] 1% acetic acid/water

gradient

35:65 to 100:0 (20 min), return to 35:65 (5 min)

flow rate

1.5 mL/min

column temp.

30 °C

detector

UV, 280 nm