P0690

Pectinase from Aspergillus niger

BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)

• Synonym(s): Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger
• CAS Number: 9032-75-1
• EC Number: 3.2.1.15 (BRENDA, IUBMB)
• EC Number: 232-885-6
• MDL number: MFCD00131809
• UNSPSC Code: 12352200

Properties

• product line: BioReagent
• form: aqueous glycerol solution
• specific activity: ≥5 units/mg protein (Lowry)
• technique(s): cell culture | plant: suitable
• application(s): agriculture
• storage temp.: 2-8°C

Description

Application

Pectinase P0690 is a natural pectinase (polygalacturonase) produced by Aspergillus niger. This enzyme breaks down pectin found in the middle lamella of plant cell walls. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

Physical form

Solution in 40% glycerol

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves