S5265

Streptolysin O from Streptococcus pyogenes

lyophilized powder, Protein ~3 % by Lowry, 25,000-50,000 U/vial

• CAS Number: 98072-47-0
• EC Number: 308-500-3
• MDL number: MFCD00132389
• UNSPSC Code: 12352202
• NACRES: NA.85

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: 25,000-50,000 U/vial
• mol wt: native 69 kDa
• composition: Protein, ~3% Lowry
• storage condition: (Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.)
• color: white
• solubility: H₂O: soluble 1 mg/mL
• antibiotic activity spectrum: neoplastics
• Mode of action: cell membrane | interferes
• storage temp.: 2-8°C
• Gene Information: Streptococcus pyogenes MGAS8232 ... slo(993428)

Description

Application

Permeabilizes membranes to permit cellular uptake of large or charged molecules.

Streptolysin O is a toxin secreted by Streptococcus pyogenes and is a prototype member of poreforming bacterial cytolysins. It is used permeabilize cell membranes to permit cellular uptake of large or charged molecules. It is used to study macromolecule delivery. It is a potential anticancer agent and is used to study suicide cancer gene therapy.

Used to create holes (reversible pores) in cholesterol-enriched plasma membranes of eukaryotic cells allowing selective permeabilization with molecules such as proteins (up to 100 kDa) and siRNAs.

Biochem/physiol Actions

Streptolysin O possesses a single polypeptide chain with a molecular weight of f62 kDa. Streptolysin O binds to membrane cholesterol and oligomerizes to create a ring structure that consists of 45 to 50 units. The ring structure inserts into the membrane to make a large pore (25 to 30 nm), which DNA, RNA, peptides and proteins may pass. It is thiol-activated. It is inhibited by allicin, an active component of garlic.

Thiol-activated toxin that permeabilizes animal cell membranes. The protein binds as a monomer to membrane cholesterol and subsequently polymerizes into large arc- and ring-shaped structures surrounding pores of >12 nm.

Thiol-activated toxin that permeabilizes animal cell membranes..

Packaging

25KU

Unit Definition

One unit will cause 50% lysis of 2% red blood cell suspensions in phosphate buffered saline, pH 7.4, after incubation at 37 °C for 30 min.

Physical form

Lyophilized powder containing Tris buffer salts, sodium azide, PMSF, and EDTA.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place

Safety Information

• Pictograms: GHS06,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H300 + H310 + H330,H315,H318,H411
• Precautionary Statements: P260 - P262 - P273 - P280 - P301 + P310 + P330 - P302 + P352 + P310 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P391 - P403 + P233
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves