T5319

Tetanolysin from Clostridium tetani

• CAS Number: 1114581-97-3
• MDL number: MFCD03458700
• UNSPSC Code: 12352200

Properties

• biological source: Clostridium tetani E88
• mol wt: 55 kDa
• application(s): cell analysis
• storage temp.: 2-8°C
• Gene Information: Clostridium tetani E88 ... CTC01888(1060232)

Description

General description

Hemolytic activity of tetanolysin is determined using 2.5% rabbit red blood cells at 37 °C for 40 min.

Application

Tetanolysin has been used to analyze the formation of lytic pores in red blood cells (RBCs)¹. It has also been used to permeabilize infected RBCs².

Biochem/physiol Actions

Cholesterol-binding toxin used to permeabilize cellular membranes to enhance the entry of macromolecules into the interior of the cell. Pores induced reported to be in the range of 20-50 nm.

Cholesterol-binding toxin used to permeabilize cellular membranes to enhance the entry of macromolecules into the interior of the cell..

Preparation Note

Prepared by a modification of the method of Haque, et al.

Reconstitution

When reconstituted with 100 μl of sterile water the concentration is 1 μg/μl in 40 mM sodium phosphate buffer, pH 7.2, containing 200 mM NaCl.

Analysis Note

Single band by SDS-PAGE.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves