HPLC Analysis of Monosaccharides Ribose, Xylitol, and Mannose on Chromolith^® NH2

column

Chromolith NH2, 10 cm x 4.6 mm I.D., 2 μm particles (1.52028.0001)

mobile phase

[A] acetonitrile; [B] water; (85:15, A:B)

flow rate

3 mL/min

pressure

47 bar (682 psi)

column temp.

23 °C

detector

UV, 190 nm

injection

5 μL

sample

ribose 5.9 mg/mL, xylitol 8.1 mg/mL, mannose 7.6 mg/mL in mobile phase