HPLC Analysis of Monosaccharides Ribose, Xylitol, and Mannose on Chromolith® NH2

CONDITIONS
column
Chromolith NH2, 10 cm x 4.6 mm I.D., 2 μm particles (1.52028.0001)
mobile phase
[A] acetonitrile; [B] water; (85:15, A:B)
flow rate
3 mL/min
pressure
47 bar (682 psi)
column temp.
23 °C
detector
UV, 190 nm
injection
5 μL
sample
ribose 5.9 mg/mL, xylitol 8.1 mg/mL, mannose 7.6 mg/mL in mobile phase
Description
Legal Information
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany