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ABE278

Sigma-Aldrich

Anti-MILI Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Piwi-like protein 2

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100 μL
$501.00

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100 μL
$501.00

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

$501.00


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biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

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This Item
SAB1300681SAB3500578HPA066880
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

saturated ammonium sulfate (SAS) precipitated

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

UniProt accession no.

Q8CDG1

UniProt accession no.

Q96J94

UniProt accession no.

Q96J94

UniProt accession no.

-

Gene Information

mouse ... Piwil2(57746)
rat ... Piwil2(306011)

Gene Information

human ... PIWIL1(9271)

Gene Information

human ... PIWIL1(9271)

Gene Information

human ... PIWIL1(9271)

General description

MILI, MIWI, and MIWI2 belong to the Piwi family of proteins. piRNAs and Piwi proteins are implicated in transposon control and are linked to transposon methylation in mammals. MILI, in particular, directly binds with piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. MILI plays an essential role in spermatogenesis, meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells.

Immunogen

KLH-conjugated linear peptide corresponding to mouse MILI.

Application

Anti-MILI Antibody is a Rabbit Polyclonal Antibody for detection of MILI also known as Piwi-like protein 2 & has been validated in WB, ICC, IHC & IP.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in male primordial germ cells. (Aravin, A.A., et al. (2008). Mol Cell. 31(6):785-799.)

Immunohistochemistry Fluoresence Analysis: A representative lot was used by an independent laboratory in embyronic testis cross sections. (Vagin, W., et al. (2009). Genes Dev. 23(15):1749-1762.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory. (Aravin, A.A., et al. (2007). Science. 316(5825):744-747.)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Western Blot in RAW264.7 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected MILI in 10 µg of RAW264.7 cell lysate.

Target description

~109 kDa observed. An uncharacterized bands at ~30, 55, and 60 kDa may be observed in some lysates.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
RAW264.7 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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