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A1418

Sigma-Aldrich

Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Mouse IgG (Fc specific)–AP

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About This Item

MDL number:
MFCD00162626
UNSPSC Code:
12352203
NACRES:
NA.46
Pricing and availability is not currently available.
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biological source

goat

Quality Level

200

recombinant

expressed in goat

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

should not react with

human

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Synthware™ vacuum or argon adapter with hose connection on side joint: ST/NS 24/40

SYNA292440

Synthware™ vacuum or argon adapter with hose connection on side

Synthware™ vacuum or argon adapter with hose connection on side joint: ST/NS 14/20

SYNA291420

Synthware™ vacuum or argon adapter with hose connection on side

Synthware™ vacuum or argon adapter with hose connection on side joint: ST/NS 19/22

SYNA291922

Synthware™ vacuum or argon adapter with hose connection on side

Synthware™ vacuum or argon adapter with hose connection on side joint: ST/NS 29/42

SYNA292942

Synthware™ vacuum or argon adapter with hose connection on side

joint

joint: ST/NS24/40

joint

joint: ST/NS14/20

joint

joint: ST/NS19/22

joint

joint: ST/NS29/42

manufacturer/tradename

Synthware A292440

manufacturer/tradename

Synthware A291420

manufacturer/tradename

Synthware A291922

manufacturer/tradename

Synthware A292942

packaging

pkg of 1 ea

packaging

pkg of 1 ea

packaging

pkg of 1 ea

packaging

pkg of 1 ea

General description

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. Anti-mouse IgG (Fc specific)–alkaline phosphatase antibody (diluted 1: 10,000 in PBS-Tween) can be used in ELISA. It is also useful in immunoblotting. Goat anti-mouse IgG (Fc specific)–alkaline phosphatase antibody reacts specifically with mouse IgG Fc fragment, IgG and its subclasses IgG1, IgG2a, IgG2b and IgG3 but not with human IgG, and mouse IgA or IgM.

Immunogen

Purified mouse IgG Fc fragment.

Application

Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37°C. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37°C.
Anti-mouse IgG (Fc specific)–alkaline phosphatase antibody can be used in ELISPOT (immunospot) assay. It can also be used in immunohistochemistry and western blot.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Preparation Note

Adsorbed to reduce background with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    087M4757V
    109M4895V
    109M4757V
    21190529
    019M4763V

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