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Key Documents

90C2

Sigma-Aldrich

Leukocyte Naphthol AS-D Chloroacetate (Specific Esterase) Kit

Synonym(s):

Leder Stain

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About This Item

UNSPSC Code:
12352106
NACRES:
NA.47

description

Select reagents packaged in gelatin capsules

Quality Level

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

dilution

(for histology)

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. With the 90C2 kit, Naphthol AS-D Chloroacetate is enzymatically hydrolyzed by "specific esterase, " liberating a free naphthol compound. This then couples with a diazonium salt, forming highly colored deposits at sites of enzyme activity. This enzyme is usually considered specific for cells of granulocytic lineage. May be used to detect neutrophils in peripheral blood, bone marrow or tissue sections which have been paraffin embedded.

Kit Components Only

Product No.
Description

  • Dimethyl formamide (9010) 25 mL

  • Fast Corinth V Salt (9015) 10 capsules

Kit Components Also Available Separately

Product No.
Description
SDS

  • 2852Acid Hematoxylin Solution 100 mLSDS

  • 905Naphthol AS-D Chloroacetate 10 x 20SDS

  • 903CTRIZMAL™ 6.3 Buffer Concentrate, pH 6.3(concentrate), 0.2 M 50 mLSDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1B

Supplementary Hazards

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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