91C

Leukocyte Naphthol AS-D Chloroacetate (Specific Esterase) Kit

• Synonym(s): Leder Stain
• UNSPSC Code: 12352106
• NACRES: NA.47

Properties

• description: Kit formulated with all liquid reagents
• Quality Level: 500
• shelf life: Expiry date on the label.
• IVD: for in vitro diagnostic use
• dilution: (for histology)
• application(s): hematology; histology
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. With the 91C kit, Naphthol AS-D Chloroacetate is enzymatically hydrolyzed by "specific esterase, " liberating a free naphthol compound. This then couples with a diazonium compound, forming highly colored deposits at sites of enzyme activity. This enzyme is usually considered specific for cells of granulocytic lineage. May be used to detect neutrophils in peripheral blood, bone marrow or tissue sections which have been paraffin embedded.

Kit Components Also Available Separately

• 915 Citrate Solution, pH 3.6±0.1 (25 °C), 27 mM 50 mL
• 912 Fast Red Violet LB Base Solution 10 mL
• 911 Naphthol AS-D Chloroacetate Solution 10 mL
• 911 Naphthol AS-D Chloroacetate Solution 50 mL

Safety Information

• Pictograms: GHS08,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H290,H302,H314,H317,H335,H360FD,H373
• Precautionary Statements: P202 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1 - Skin Sens. 1 - STOT RE 2 Oral - STOT SE 3
• Target Organs: Kidney, Respiratory system
• Supplementary Hazards: EUH019
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects