C4749

Carboxylesterase 2 human

recombinant, expressed in mouse NSO cells, ≥95% (SDS-PAGE)

• Synonym(s): CES2, CES2A1
• EC Number: 3.1.1.1 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in mouse NSO cells
• Quality Level: 200
• Assay: ≥95% (SDS-PAGE)
• form: solution
• specific activity: ≥1.0 EU/μg; 30,000 pmol/min-μg protein
• mol wt: predicted mol wt ~60 kDa
• concentration: 0.4-0.6 mg/mL
• impurities: ≤1.0 EU/μg endotoxin
• NCBI accession no.: NM_003869; NP_003860
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CES2(8824)

Description

Biochem/physiol Actions

Human carboxylesterase 2 (hCE-2) recognizes a substrate with a large alcohol group and small acyl group. Its substrate specificity may be restricted by a capability of acyl-hCE-2 conjugate formation due to the presence of conformational interference in the active site pocket. Carboxylesterases catalyze the biotransformation of several ester-containing drugs and prodrugs such as angiotensin-converting enzyme inhibitor (temocarpil, cilazapril), anti-tumor drugs (capecitabin) and narcotics.

Member of a serine esterase family that hydrolyze ester and amide bonds. Carboxylesterase 2 is an endoplasmic reticulum-bound hydrolase that plays a critical role in xenobiotic detoxification and activation for ester-containing therapeutics. Carboxylesterase 2 is also involved in the detoxification of drugs such as heroin and cocaine. This enzyme is thought to play a role in lipid metabolism.

Unit Definition

One unit will cause the hydrolysis of 1 picomole of p-nitrophenylacetate per minute at pH 7.5 at 25 deg C.

Physical form

Supplied as a solution containing sodium chloride, sodium acetate, and 20% glycerol.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable