G0774

Glycerokinase from Bacillus stearothermophilus

buffered aqueous solution, ≥75 units/mg protein (biuret)

• Synonym(s): ATP:glycerol 3-phosphotransferase, Glycerol Kinase
• CAS Number: 9030-66-4
• EC Number: 2.7.1.30 (BRENDA, IUBMB)
• EC Number: 232-862-0
• MDL number: MFCD00131205
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Properties

• biological source: Bacillus sp. (Bacillus steaarothermophilus)
• Quality Level: 200
• Assay: ≥5.0 mg protein/mL (biuret)
• form: buffered aqueous solution
• specific activity: ≥75 units/mg protein (biuret)
• storage condition: dry at room temperature
• color: beige
• application(s): life science and biopharma
• storage temp.: 2-8°C
• Gene Information: Bacillus sp. ... glpK(89613566)

Description

General description

Research area: Cell Signaling

Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.

Application

Glycerokinase from Bacillus stearothermophilus has been used in steady-state kinetics studies.

Biochem/physiol Actions

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.Oxidized glycerol-3-phosphate results in dihydroxyacetone phosphate which enters either glycolysis or gluconeogenesis. Elevated glycerol is observed in glycerol kinase deficiency leading to pseudohypertriglyceridemia.

Unit Definition

One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.

Physical form

Stabilized solution in Tris buffer, pH 7.3

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves