G4509

Glycerokinase from Escherichia coli

lyophilized powder, 40-100 units/mg protein

• Synonym(s): ATP:glycerol 3-phosphotransferase, Glycerol Kinase
• CAS Number: 9030-66-4
• EC Number: 2.7.1.30 (BRENDA, IUBMB)
• EC Number: 232-862-0
• MDL number: MFCD00066766
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Escherichia coli
• Quality Level: 200
• form: lyophilized powder
• specific activity: 40-100 units/mg protein
• composition: Protein, ≥50% biuret
• foreign activity: Triose-phosphate isomerase, hexokinase, myokinase, glycerol dehydrogenase and NADH oxidase ≤0.05%
• storage temp.: −20°C

Description

Application

Glycerol kinase (glpK) was used to study the effects of pain controlling neuropeptides on human fat cell lipolysis.

Biochem/physiol Actions

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. <<<20,21>>> It is also subject to feedback regulation by fructose-1,6-bisphosphate.<<<22>>>

Unit Definition

One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.

Physical form

Partially purified lyophilized powder, balance is primarily salts and EDTA.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves