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MTOXCE1A2

Sigma-Aldrich

CypExpress 1A2 Cytochrome P450 human

Synonym(s):

CP12, CYP1A2, P3-450, P450 1A2, P450(PA)

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200 μL
MYR 2,199.00

MYR 2,199.00


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200 μL
MYR 2,199.00

About This Item

EC Number:
UNSPSC Code:
41106200
NACRES:
NA.77

MYR 2,199.00


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biological source

human

recombinant

expressed in Pichia pastoris

form

dry powder

UniProt accession no.

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CYP1A2(1544)

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biological source

human

biological source

human

biological source

human

biological source

human

recombinant

expressed in Pichia pastoris

recombinant

expressed in Pichia pastoris

recombinant

expressed in Pichia pastoris

recombinant

expressed in Pichia pastoris

form

dry powder

form

dry powder

form

dry powder

form

dry powder

Gene Information

human ... CYP1A2(1544)

Gene Information

human ... CYP2C9(1559)

Gene Information

human ... CYP2D6(1565)

Gene Information

human ... CYP1A1(1543)

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

UniProt accession no.

P05177

UniProt accession no.

P11712

UniProt accession no.

P10635

UniProt accession no.

-

Biochem/physiol Actions

Cytochrome P450 enzymes are heme containing monooxygenases which are found primarily in the mammalian liver. They catalyze the oxidative metabolism of xenobiotics.[1][2][3][4] This metabolism is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants from the body. Furafylline and α-naphthoflavone are inhibitors, whereas omeprazole and lansoprazole are inducers of the enzyme. The isozyme CYP1A2 is a major pathway for the in vivo conversion of phenacetin into acetaminophen via O-deethylation. This isozyme also catalyzes the 5-hydroxylation of thiabendazole.
Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids)[4] and to detoxify exogenous compounds (drugs or toxins).[3] Some isoforms have narrow substrate specificity, while others are promiscuous.

Features and Benefits

CypExpress 1A2 is a permeabilized and stabilized dried yeast powder preparation containing full length, unmodified, human CYP 1A2 and recombinant human NADPH oxidoreductase. Using proprietary processes, CypExpress are expressed in Pichia yeast. Following optimal expression, the cells are inactivated, permeabilized and dried to a fine powder. CypExpress retains the cellular mechanisms to provide the P450 enzymes with the energy and cofactors to continue to function for long experiments, and can generate larger amounts of metabolite than mammalian microsomes or other genetically engineered expression systems. It is designed for rapid screening of drug metabolites and for scaling up metabolite generation.

Unit Definition

A reaction mixture containing 500 μM phenacetin and 100 mg/mL of CypExpress 1A2 will produce 35 μM acetaminophen in 4 hours at 30°C under the conditions described.

Physical form

Light yellow, very fine powder

Reconstitution

Per Protocol/Variable

Legal Information

CypExpress is a trademark of Oxford Biomedical Research

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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