S2563

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp.

• Synonym(s): SCDase
• MDL number: MFCD02100603
• UNSPSC Code: 12352211
• NACRES: NA.32

Properties

• foreign activity: exoglycosidases: α- and β-galactosidase, α- and β-N-acetylgalactosaminidase, β-N-acetylglucosaminidase, α-mannosidase, α-fucosidase, and sialidase., essentially free; protease and sphingomyelinase, essentially free
• Quality Level: 200
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. is a hydrolytic enzyme.

Application

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. has been used in enzymatic conversion and derivatization of sulfatides. It has also been used to hydrolyze the fatty acid chain of mactosyl ceramide.

Biochem/physiol Actions

Hydrolyzes the N-acyl linkage between fatty acids and sphingosines in ceramides of various sphingolipids.

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp., under certain conditions, can recyclate lyso-sphingolipids. It condenses fatty acids to sphingosine to generate ceramide. The enzyme mainly acts on neutral and acidic glycosphingolipids.

Unit Definition

One unit will hydrolyze 1 μmol of asialo GM₁ per minute at pH 6.0 at 37 °C.

Physical form

Solution in 50 mM sodium acetate, pH 6.0, with 0.1% Lubrol PX.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable