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SAB4200715

Sigma-Aldrich

Anti-β-tubulin antibody, Mouse monoclonal

clone 2-28-33, purified from hybridoma cell culture

Synonym(s):

Anti-TUBB1

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100 μL
$731.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

$731.00

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biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

2-28-33, monoclonal

form

buffered aqueous solution

mol wt

~50 kDa

species reactivity

dog, chicken, rat, bovine, monkey, human, mouse

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.5 μg/mL using mouse embryo fibroblast NIH-3T3 cells extract
immunofluorescence: 1-2 μg/mL using human foreskin fibroblast HS68 cells
immunohistochemistry: suitable

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Anti-β-tubulin antibody, Mouse monoclonal clone 2-28-33, purified from hybridoma cell culture

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Anti-α-Tubulin antibody, Mouse monoclonal

Anti-β-Tubulin−FITC antibody, Mouse monoclonal clone TUB 2.1, purified from hybridoma cell culture

F2043

Anti-β-Tubulin−FITC antibody, Mouse monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

clone

2-28-33, monoclonal

clone

TUB-1A2, monoclonal

clone

B-5-1-2, monoclonal

clone

TUB 2.1, monoclonal

species reactivity

dog, chicken, rat, bovine, monkey, human, mouse

species reactivity

Xenopus, Arabidopsis sp., yeast, insect, rat, hamster, porcine, monkey, bovine, human, mouse, canine, chicken

species reactivity

mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat

species reactivity

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

Gene Information

human ... TUBB(203068)
mouse ... Tubb3(22152)

Gene Information

human ... TUBA1A(7846)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Gene Information

human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383)
mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb3(22152)
rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

General description

Anti-β-tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 2-28-33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin also known as TUBB is the major building block of microtubules. Microtubules assemble from heterodimers of α-tubulin and β-tubulin; both have a molecular weight of ~ 50 kDa and share considerable homology. For β-tubulin, five evolutionarily conserved isotypes have been identified, which are regularly utilized in the same cell types of different species.

Immunogen

Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Application

Monoclonal Anti-β-tubulin has been used:
  • in immunoblotting
  • in immunofluorescence
  • in immunohistochemistry

Biochem/physiol Actions

Monoclonal Anti-β-tubulin specifically recognizes the two major β-tubulin isotypes and one of the minor β-tubulin isotypes of the free-living soil nematode Caenorhabditis elegans. It also binds to Sarkosyl resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, neuronal axons of the larva of Ciona intestinalis (ascidian), microtubules in root tip cells in Arabidopsis thaliana and to human, mouse, rat, monkey, bovine, dog and chicken β-tubulin. The different β-tubulin isotypes are suggested to differ from each other in their ability to polymerize into microtubules.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Lot/Batch Number
    096M4782V

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