SML2239

INF39

≥98% (HPLC)

• Synonym(s): 2-Chloro-α-methylene-benzenepropanoic acid ethyl ester, Ethyl 2-(2-chlorobenzyl)acrylate
• Empirical Formula (Hill Notation): C₁₂H₁₃ClO₂
• CAS Number: 866028-26-4
• Molecular Weight: 224.68
• MDL number: MFCD27930542
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Assay: ≥98% (HPLC)
• form: oil
• color: colorless to light brown
• storage temp.: −20°C
• SMILES string: ClC1=C(CC(C(OCC)=O)=C)C=CC=C1
• InChI: 1S/C12H13ClO2/c1-3-15-12(14)9(2)8-10-6-4-5-7-11(10)13/h4-7H,2-3,8H2,1H3
• InChI key: VTAOWWAFBSFWSG-UHFFFAOYSA-N

Description

Biochem/physiol Actions

INF39 is an orally active acrylate derivative and a non-cytotoxic INF4E analog (no toxicity at 100 μM in THP-1 cultures vs. TC50 = 65 μM with INF4E) that acts as an irreversible inhibitor against NLRP3 (NACHT, LRR and PYD domains-containing protein 3) ATPase activity (52% inhibition in 15 min by 100 μM INF39; 105 ng human NLP3 & 250 μM ATP) essential for the NLRP3 inflammasome assembly and activation. INF39 effectively decreases 5 mM (30 min) ATP-induced interleukin-1β (IL-1β) release and pyroptosis of murine bone marrow-derived macrophages in cultures (by 55-58% and 43-65%, respectively, with 1-hr 10 μM INF39 pretreatment of LPS-primed BMDM) and alleviates gut-associated inflammation in a rat model of 2,4-dinitrobenzenesulfonic acid (DNBS)-induced colitis in vivo (12.5-50 mg/kg/day in 0.2 mL olive oil p.o.).

Orally active irreversible NLRP3 inhibitor that suppresses ATP-dependent macrophage activation and alleviates DNBS-induced colitis in rats.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable