T2391

Triosephosphate Isomerase from rabbit muscle

Type III-S, ammonium sulfate suspension, ≥4,000 units/mg protein

• Synonym(s): D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI
• CAS Number: 9023-78-3
• EC Number: 5.3.1.1 (BRENDA, IUBMB)
• MDL number: MFCD00082080
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: rabbit muscle
• Quality Level: 200
• type: Type III-S
• form: ammonium sulfate suspension
• specific activity: ≥4,000 units/mg protein
• mol wt: calculated mol wt 53.2 kDa
• foreign activity: 3-phosphoglyceric phosphokinase ≤0.01%; glyceraldehyde-3-phosphate dehydrogenase ≤0.01%; pyruvate kinase, lactic dehydrogenase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase and aldolase ≤0.001%
• storage temp.: 2-8°C

Description

Application

Triosephosphate isomerase has been used in a study to assess molecular characterizations of Cryptosporidium, Giardia, and Enterocytozoon. Triosephosphate isomerase has also been used in a study to investigate apoptosis of hepatocellular carcinoma cell lines.

Unit Definition

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Physical form

Crystalline suspension in 3.2 M (NH₄)₂SO₄ solution, pH 6.0

Analysis Note

Protein determined by biuret

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves