T2507

Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

• Synonym(s): D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI
• CAS Number: 9023-78-3
• EC Number: 5.3.1.1 (BRENDA, IUBMB)
• EC Number: 232-774-2
• MDL number: MFCD00082080
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type I
• Quality Level: 200
• form: ammonium sulfate suspension
• specific activity: ~10,000 units/mg protein
• mol wt: 53 kDa by equilibrium centrifugation
• foreign activity: pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%
• storage temp.: 2-8°C

Description

Application

Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins.

Unit Definition

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Physical form

Crystalline suspension in 2.7 M (NH₄)₂SO₄, 0.5 mM EDTA, pH 6.5

Analysis Note

Protein determined by biuret.

Other Notes

Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves