U3383

Urease Buffer Reagent

• Synonym(s): Urea colorimetric determination assay reagent
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• description: When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.
• Quality Level: 200
• form: powder
• mol wt: 545 kDa
• storage temp.: 2-8°C

Description

General description

Urease is a 545 kDa protein critical for the hydrolysis of urea to carbon dioxide and ammonia.

Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide. It is secreted by several bacterial species, invertebrates, fungi, and plants. Detection of urease activity is a potential diagnostic tool for Helicobacter pylori infections and several oral bacterial infections. two Ni²⁺ ions bridged by a hydroxyl group and a carbamylated lysine.

Application

Urease Buffer Reagent has been used:

• to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside method
• in cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cells
• to study the metabolic capacity of rat hepatocytes

Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H315 - H319 - H334
• Precautionary Statements: P261 - P264 - P280 - P284 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves