126575

Albumin, Bovine Serum, Fraction V, Fatty Acid-Free

• Synonyme(s) : Albumin, Bovine Serum, Fraction V, Fatty Acid-Free
• CAS Number: 9048-46-8
• Numéro MDL: MFCD00797675
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.25

Propriétés

• Description: Merck USA index - 14, 8468
• Essai: ≥95% (cellulose acetate electrophoresis)
• Forme: powder
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: ≤0.02% fatty acids; ≤3% ash; ≤5% moisture
• Couleur: white to light yellow; light yellow to light brown
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

Albumin, Bovine Serum, (BSA) Fraction V, Fatty Acid-Free is useful for immunodiagnostic assays. It is designed for use in serological testing, radioimmunoassay, and hormone response studies. It is also applicable in binding and transport studies. BSA is prepared by cold ethanol extraction (Cohn method) with a pH: 6.8-7.4.

Bovine serum albumin (BSA) is a non-glycosylated globular, α-helical protein belonging to the serum albumin family. It consists of three domains with two sub-domains each and has 17-disulfide bonds.

Application

Albumin, Bovine Serum, Fraction V, Fatty Acid-Free has been used:

• as a component of: blocking buffer immunoelectron microscopy (IEM) for various cells
• mitochondrial isolation buffer (MIB) and mitochondria assay solution (MAS) to trap free fatty acids released during adipose tissue homogenization
• blocking buffer for wholemount staining of the esophageal epithelium (EE) in immunofluorescence

Actions biochimiques/physiologiques

Bovine Serum Albumin (BSA), the most vital component of cell culture media, aids in embryonic stem cells (hESC) differentiation. It aids the transportation of drugs, hormones, and fatty acids. BSA is also used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Avertissement

Toxicity: Standard Handling (A)

Notes préparatoires

May take up to 24 h to fully dissolve.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable