126579

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free

• Synonyme(s) : Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free
• CAS Number: 9048-46-8
• Numéro MDL: MFCD00130384
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.25

Propriétés

• Description: Merck USA index - 14, 8468
• Essai: ≥95% (Albumin, electrophoresis)
• Forme: lyophilized powder
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Technique(s): immunofluorescence: suitable
• Impuretés: ≤0.2% fatty acids; ≤2.0% Ash; ≤5% moisture; ≤50 EU/mg endotoxins
• Couleur: pale green
• Solubilité: aqueous buffer: 20 mg/mL; water: 20 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free is designed for use in cell culture systems and animal perfusion studies. It has a pH of 7.0 (1% solution).

Bovine serum albumin (BSA), a member of the serum albumin family, contains three domains and two sub-domains under each. It is a non-glycosylated globular, α-helical protein that has 17-disulfide bonds.

Application

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free has been used in the preparation of advanced glycation end-products (AGEs). It has also been used to dilute lipopolysaccharide (LPS) and tumor necrosis factor (TNF) and in the stimulation and fixation of whole blood cells.

Actions biochimiques/physiologiques

Bovine Serum Albumin (BSA) is the most important constituent of cell culture media. It aids embryonic stem cells (hESC) differentiation. BSA facilitates the transport of drugs, hormones, and fatty acids. It elicits high solubility and is suitable in the cosmetic and pharmaceutical industries. It is applicable as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Avertissement

Toxicity: Standard Handling (A)

Notes préparatoires

Material may take up to 24 h to fully dissolve.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable