38185
Quantification kit for oxidized and reduced glutathione
200 tests
Synonyme(s) :
γ-L-glutamyl-L-cysteinylglycine kit, Quantification kit
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About This Item
Code UNSPSC :
12352108
Nomenclature NACRES :
NA.32
Produits recommandés
Catégories apparentées
Description générale
Glutathione Quantification Kit is designed to detect oxidized and reduced Glutathione. Glutathione is usually represented in a reduced form called GSH; however, GSH is converted into its oxidized state (GSSG) upon oxidative stress. The ratio between GSH and GSSG is considered an index for oxidative stress. Glutathione, also known as γ-L-glutamyl-L-cysteinylglycine, is a tripeptide compound that is involved in anti-oxidation and drug metabolism. It acts as a substrate for enzymes like glutathione peroxidase, glutathione S-transferase, and thiol transferase.
Application
Glutathione Quantification Kit is used to detect the total amount of glutathione as an index for oxidative stress or disease risk.
Caractéristiques et avantages
The detection ranges of total glutathione and GSSG using in this kit are from 0.5 μmol/l to 50 μmol/l and from 0.5 μmol/l to 25 μmol/l, respectively.
Composants
Glutathione Quantification Kit contains:
- Enzyme Solution (50µl X 1)
- Buffer Solution (60ml X 1)
- Standard GSH (X 1)
- Masking Reagent (20µl X 1)
- Co-enzyme (X 2)
- Substrate (DTNB) (X4)
- Standard GSSG (X 1)
Remarque sur l'analyse
Glutathione Quantification Kit contains a GSH masking reagent. This enables GSSG detection in the sample by measuring the absorption (λmax = 412 nm) obtained from a colorimetric reaction of DTNB (5,5’-dithiobis (2-nitrobenzoic acid)) coupled with the enzymatic recycling system. The total quantity of GSH can be measured by subtracting the amount of GSSG from the total amount of glutathione.
Autres remarques
not available in Korea, Singapore, Taiwan and Japan
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - Skin Sens. 1
Code de la classe de stockage
3 - Flammable liquids
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