47608

Formaldéhyde solution

BioReagent, ≥36.0% in H₂O (T)

• Synonyme(s) : Formol
• Formule linéaire : HCHO
• CAS Number: 50-00-0
• Poids moléculaire : 30.03
• Beilstein: 1209228
• Numéro MDL: MFCD00003274
• Code UNSPSC : 12352114
• ID de substance PubChem : 329757328
• Nomenclature NACRES : NA.52

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 100
• Densité de vapeur: 1.03 (vs air)
• Pression de vapeur: 52 mmHg ( 37 °C)
• Gamme de produits: BioReagent
• Forme: liquid
• Température d'inflammation spontanée: 572 °F
• Contient: ~10% methanol as stabilizer
• Concentration: ≥36.0% in H₂O (T); 36.0-38.0% (aldehyde method)
• Technique(s): electrophoresis: suitable
• Impuretés: ≤0.03% free acid (as HCOOH)
• Résidus de calcination: ≤0.05% (as SO₄)
• pH: 2.8-4.0
• Densité: 1.09 g/mL at 20 °C; 1.09 g/mL at 25 °C (lit.)
• Traces d'anions: chloride (Cl^-): ≤10 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• Traces de cations: Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Na: ≤100 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Zn: ≤1 mg/mL
• λ: 2 M in H₂O
• Absorption UV: λ: 260 nm A_max: 0.01; λ: 280 nm A_max: 0.01
• Adéquation: in accordance for electrophoresis of RNA in agarose gels
• Température de stockage: room temp
• Chaîne SMILES: [H]C([H])=O
• InChI: 1S/CH2O/c1-2/h1H2
• Clé InChI: WSFSSNUMVMOOMR-UHFFFAOYSA-N

Description

Description générale

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.
It is an aliphatic aldehyde that is widely used in industrial applications for the synthesis of several chemicals such as melamine formaldehyde, urea-formaldehyde, phenol formaldehyde and industrial resins, paraformaldehyde, polyols, chelating agents, slow release fertilizers, 1,4-butanediol and hexamethylenetetramine.

Application

Formaldehyde solution is suitable for:

• denaturation and separation of RNA molecules
• anti-bacterial treatment
• fixation of cells

Formaldehydesolution has been used:

• in the fixation of human spermatogonial stem cell transplantation (SSCT).
• to crosslink T- cell acute lymphoblastic leukemia protein/DNA complex during chromatin immunoprecipitation.
• in fixing zebra fish larvae for neutrophil staining.

Attention

may contain precipitate (paraformaldehyde)

Principe

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

Informations sur la sécurité

• Pictogrammes: GHS02,GHS06,GHS08,GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H226,H301 + H311,H314,H317,H330,H335,H341,H350,H370
• Conseils de prudence: P202 - P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
• Organes cibles: Eyes,Central nervous system, Respiratory system
• Code de la classe de stockage: 3 - Flammable liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 133.0 °F - closed cup
• Point d'éclair (°C): 56.11 °C - closed cup
• Équipement de protection individuelle: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter