59767

Chlorophenol Red-β-D-galactopyranoside

β-galactosidase, ≥90% (HPLC), powder or crystals

• Synonyme(s) : CPRG, Chlorophenol Red-β-D-galactoside
• Formule empirique (notation de Hill) : C₂₅H₂₂Cl₂O₁₀S
• CAS Number: 99792-79-7
• Poids moléculaire : 585.41
• Numéro MDL: MFCD05863726
• Code UNSPSC : 12352204
• ID de substance PubChem : 57651696
• Nomenclature NACRES : NA.83

Propriétés

• Product Name: Chlorophenol Red-β-D-galactopyranoside, ≥90% (HPLC)
• Essai: ≥90% (HPLC)
• Forme: powder or crystals
• Solubilité: water: 20 mg/mL, clear, orange to very deep red
• Température de stockage: −20°C
• Chaîne SMILES: OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)\c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1
• Clé InChI: YOUWVNCQJOMMEU-AETRGLENSA-N

Description

Description générale

Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength yellow dye commonly used for colorimetric assays. It serves as a substrate for β-galactosidase (β-gal) which hydrolyzes CPRG to form chlorophenol red (purple colored). This product is quantified by measuring its absorbance at 570 nm. CPRG is used to identify fecal coliform bacteria. It is about 10 times more sensitive than other chromogenic agents such as o-nitrophenyl-β-galactoside (ONPG). CPRG also has a high rapid turnover.

Application

Chlorophenol Red-β-D-galactopyranoside has been used as a substrate for β-galactosidase (β-gal):

• in paper-based cell-free protein expression system for target RNA detection
• in colorimetric detection of multiple clinically relevant mutations using a lacZ output gene
• in colorimetric assay for bacteria quantification

Autres remarques

Measures the expression levels of β-galactosidase in transfected cells. The lacZ gene is a popular reporter gene in transfection reactions because its product, β-galactosidase, is a very stable enzyme.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)