73144

Resorufin

95%

• Synonyme(s) : 7-Hydroxy-3H-phenoxazin-3-one, NSC 12097
• Formule empirique (notation de Hill) : C₁₂H₇NO₃
• CAS Number: 635-78-9
• Poids moléculaire : 213.19
• Beilstein: 174850
• Numéro CE : 211-241-8
• Numéro MDL: MFCD00128991
• Code UNSPSC : 12171500
• ID de substance PubChem : 329764655
• Nomenclature NACRES : NA.32

Propriétés

• Niveau de qualité: 100
• Essai: ≥95.0% (HPLC); 95%
• Forme: solid
• Pf: >300 °C (lit.)
• Température de stockage: −20°C
• Chaîne SMILES: O=C(C=C1)C=C2C1=NC3=CC=C(O)C=C3O2
• InChI: 1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H
• Clé InChI: HSSLDCABUXLXKM-UHFFFAOYSA-N

Description

Description générale

Red fluorophore standard. Widely used in enzyme assay systems. Absorption and fluorescence of resorufin are pH dependent. Below the pKa (~6.0), Abs. shifts to ~ 480 nm and both E and fluorescence quantum yield are markedly lower. Resorufin is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol.

Resorufin has high extinction coefficient and good chemical and photostability. These properties of resorufin make it perfect for its usage in inverse fluorescence assay to detect H₂O₂ by measuring the disappearance of its fluorescence upon horseradish peroxidase-mediated oxidation to a nonfluorescent derivative.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H315,H319,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable