A1908

Asialofetuin from fetal calf serum

Type II (Sigma designation)

• Numéro MDL: MFCD00130561
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.61

Propriétés

• Source biologique: bovine
• Type: Type II (Sigma designation)
• Forme: powder
• Technique(s): activity assay: suitable; inhibition assay: suitable
• Impuretés: salt, essentially free; ≤1.0% N-acetylneuraminic acid
• Solubilité: H₂O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow
• Température de stockage: 2-8°C

Description

Application

Asialofetuin from fetal calf serum is suitable for use:

• in the assay of the lactose binding activity of the galectin-7 by immunodetection of in situ binding activity to asialofetuin immobilized on nitrocellulose
• in the inhibition of zona pellucida hardening of mouse oocytes
• as an acceptor in sialyltransferase enzyme activity assay

Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.

Actions biochimiques/physiologiques

Asialofetuin (AF) binds to asialoglycoprotein receptor (ASGP-R) and is employed as a ligand for drug delivery to the hepatocytes. It aids serum-resistant lipoplexes for efficient gene deliver into the HepG2 (human hepatoblastoma) cells.

Feutins are glycoproteins that belong to the cystatin superfamily. They are characterized by the presence of two tandemly arranged cystatin-like domains and an unrelated domain. It binds to transforming growth factor (TGF)-β/BMP (bone morphogenic protein) cytokines.

Notes préparatoires

Enzymatic cleavage using neuraminidase

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)