A2263

α-Amanitin

from Amanita phalloides, ≥85% (HPLC), powder

• Formule empirique (notation de Hill) : C₃₉H₅₄N₁₀O₁₄S
• CAS Number: 23109-05-9
• Poids moléculaire : 918.97
• Beilstein: 1071138
• Numéro CE : 245-432-2
• Numéro MDL: MFCD00215842
• Code UNSPSC : 12352202
• ID de substance PubChem : 24890639
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: Amanita phalloides
• Niveau de qualité: 200
• Essai: ≥85% (HPLC)
• Forme: powder
• Poids mol.: 918.97 g/mol
• Couleur: white to light yellow
• Pf: 254-255 °C (lit.)
• Solubilité: H₂O: 1.0 mg/mL
• ε (coefficient d'extinction): 13,500 at 310 nm in H₂O at 1 M
• Température de stockage: −20°C
• Chaîne SMILES: CCC(C)C1NC(=O)CNC(=O)C2Cc3c([nH]c4cc(O)ccc34)S(=O)CC(NC(=O)CNC1=O)C(=O)NC(CC(N)=O)C(=O)N5CC(O)CC5C(=O)NC(C(C)C(O)CO)C(=O)N2
• InChI: 1S/C39H54N10O14S/c1-4-16(2)31-36(60)42-11-29(55)43-25-15-64(63)38-21(20-6-5-18(51)7-22(20)46-38)9-23(33(57)41-12-30(56)47-31)44-37(61)32(17(3)27(53)14-50)48-35(59)26-8-19(52)13-49(26)39(62)24(10-28(40)54)45-34(25)58/h5-7,16-17,19,23-27,31-32,46,50-53H,4,8-15H2,1-3H3,(H2,40,54)(H,41,57)(H,42,60)(H,43,55)(H,44,61)(H,45,58)(H,47,56)(H,48,59)
• Clé InChI: CIORWBWIBBPXCG-UHFFFAOYSA-N

Description

Amino Acid Sequence

Asn-Hyp-Hyi-Trp-Gly-Ile-Gly-Cys [Bridge: 4-8]

Application

α-Amanitin has been used:

• for inducing transcriptional arrest in NT2 cells prior to immunofluorescence assay
• to induce nephrotoxicity in mice renal tissues
• to induce and analyse genotoxicity in mice bone marrow cells by cell viability assay, comet assay and chromosomal aberration assay

Actions biochimiques/physiologiques

Inhibits eukaryotic RNA polymerase II and III; does not inhibit RNA polymerase I or bacterial RNA polymerase; inhibits mammalian protein synthesis.

The major toxic constituent of the mushroom, Amanita phalloides, inhibits eukaryotic RNA polymerase II and III, but does not inhibit RNA polymerase I or bacterial RNA polymerase. Inhibits mammalian protein synthesis.

Attention

When stored frozen in the dark, retained samples were found to be greater than 99% by HPLC after two years.

Reconstitution

Store a 1 mg/ml aqueous stock solution frozen at -20°C. It does not degrade with repeated freeze-thaw cycles. Dilute into the appropriate buffer immediately prior to use. Product is destroyed by concentrated acid or base.

Informations sur la sécurité

• Pictogrammes: GHS06,GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H300,H373
• Conseils de prudence: P260 - P264 - P270 - P301 + P310 - P314 - P405
• Classification des risques: Acute Tox. 1 Oral - STOT RE 2
• Organes cibles: Kidney,Blood,Liver,Gastrointestinal tract,Respiratory Tract,Central nervous system
• Code de la classe de stockage: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges