A4487

Aphidicolin, Ready Made Solution

from Nigrospora sphaerica

• Synonyme(s) : ICI 69653, NSC-234714
• Formule empirique (notation de Hill) : C₂₀H₃₄O₄
• CAS Number: 38966-21-1
• Poids moléculaire : 338.48
• Numéro CE : 200-664-3
• Numéro MDL: MFCD00083214
• Code UNSPSC : 51102829
• Nomenclature NACRES : NA.85

Propriétés

• Source biologique: Nigrospora sphaerica
• Niveau de qualité: 200
• Pression de vapeur: 0.55 hPa ( 20 °C)
• Forme: liquid
• Couleur: clear colorless
• Solubilité: H₂O: miscible (completely)
• Spectre d'activité de l'antibiotique: neoplastics; viruses
• Mode d’action: DNA synthesis | interferes; enzyme | inhibits
• Température de stockage: −20°C
• Chaîne SMILES: O[C@]1([C@H]2C[C@@]3([C@@]4([C@H]([C@@]([C@@H](CC4)O)(CO)C)CC[C@H]3C2)C)CC1)CO
• InChI: 1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1
• Clé InChI: NOFOAYPPHIUXJR-APNQCZIXSA-N

Description

Description générale

Aphidicolin is a tetracyclic diterpene with antiviral and antimitotic properties used for cell cycle synchronization in various cell lines.

Application

Aphidicolin, from Nigrospora sphaerica, was used for cell cycle synchronization of tobacco BY-2 cells. It is used to block the entry of cells into S-phase.

Actions biochimiques/physiologiques

Aphidicolin specifically inhibits DNA polymerase α that is responsible for DNA replication. It also inhibits α-like DNA polymerases of plants and yeasts but does not inhibit synthesis of RNA and proteins. Aphidicolin competes for the dCTP-specific binding site on DNA polymerase α specifically. Aphidicolin has antiviral and antimitotic properties. It blocks the cell cycle at S phase. It has been shown to induce apoptosis in HeLa cells.

Conditionnement

1 ml

Forme physique

Aphidicolin Ready Made Solution supplied as a 1 mg/ml solution in DMSO.

Autres remarques

Keep container tightly closed in a dry and well-ventilated place. Storage class (TRGS 510): Combustible liquids

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): 188.6 °F - closed cup
• Point d'éclair (°C): 87 °C - closed cup