A7653

L-Alanine Dehydrogenase from Bacillus subtilis

buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

• Synonyme(s) : L-Alanine: NAD⁺ oxidoreductase (deaminating)
• CAS Number: 9029-06-5
• Numéro CE : 1.4.1.1 (BRENDA, IUBMB)
• Numéro CE : 232-847-9
• Numéro MDL: MFCD00131441
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Bacillus subtilis
• Niveau de qualité: 200
• Forme: buffered aqueous glycerol solution
• Activité spécifique: ~30 units/mg protein (Lowry)
• Activité étrangère: LDH ~1% (using pyruvate as substrate)
• Température de stockage: −20°C

Description

Application

L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection .

Actions biochimiques/physiologiques

L-Alanine dehydrogenase is an A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.

Définition de l'unité

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH₃ per min at pH 10.0 at 25 °C.

Forme physique

Solution in 50% glycerol containing 10 mM potassium phosphate buffer, pH 7.7

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)