G2626

L-Glutamic Dehydrogenase from bovine liver

Type II, 50% glycerol solution, ≥35 units/mg protein

• Synonyme(s) : L-GLDH, L-Glutamate:NAD[P]⁺ Oxidoreductase (deaminating), Glutamate Dehydrogenase from bovine liver
• CAS Number: 9029-12-3
• Numéro CE : 1.4.1.3 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131461
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bovine liver
• Niveau de qualité: 200
• Type: Type II
• Forme: glycerol solution (50%)
• Activité spécifique: ≥35 units/mg protein
• Poids mol.: 310-350 kDa
• Numéro d'accès UniProt: P00366
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Informations sur le gène: cow ... GLUD1(281785)

Description

Application

L-glutamic dehydrogenase was used to catalyze the conversion of isocitrate into α-ketoglutarate and carbon dioxide.

Actions biochimiques/physiologiques

Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species.

The bovine enzyme is characterized by three sets of properties:

• It has a reversible concentration-dependent association, producing higher molecular weight forms.
• Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates.
• Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

Définition de l'unité

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.

Forme physique

50% glycerol solution

Remarque sur l'analyse

Protein determined by biuret.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable