C0278

Chloroperoxidase from Caldariomyces fumago

buffered aqueous suspension, ≥3,000 units/mL

• Synonyme(s) : Chloride Peroxidase, Chloride:hydrogen-peroxide oxidoreductase
• CAS Number: 9055-20-3
• Numéro CE : 1.11.1.10 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130774
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: buffered aqueous suspension
• Niveau de qualité: 200
• Poids mol.: 42 kDa
• Concentration: ≥3,000 units/mL
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Chloroperoxidase is a heme containing glycoprotein that is secreted from fungus. Chloroperoxidase (CPO) is a extracellular heme glycoenzyme containing ferriprotoporphyrin IX as the prosthetic group.

Application

A useful alternative to lactoperoxidase for ¹³¹I ion labeling studies, for bromination of proteins, and for ³⁶Cl labeling of macromolecules in long-term isolation procedures.

Chloroperoxidase from Caldariomyces fumagois is useful alternative to lactoperoxidase for ¹³¹I ion labeling studies, for bromination of proteins, and for ³⁶Cl labeling of macromolecules in long-term isolation procedures. It has been used to study biocatalytic oxidation in polymersome nanoreactors .

It has been used to study biocatalytic oxidation in polymersome nanoreactors.

Actions biochimiques/physiologiques

Chloroperoxidase (CPO) is secreted from fungus and exhibits a broad spectrum of chemical reactivities. It is a peroxide-dependent chlorinating enzyme and it also catalyzes peroxidase, catalase and cytochrome P450-type reactions of dehydrogenation, hydrogenperoxide (H₂O₂) decomposition and oxygen insertion, respectively. The enzyme has magnetic and spectroscopic properties similar to that of cyctochrome P-450. CPO from the fungus Caldariomyces fumago has the capacity to chlorinate aromatic hydrocarbons, including polycyclic aromatic hydrocarbons (PAHs). PAHs are considered to be a potential health risk because of their possible carcinogenic and mutagenic activities and are widely dispersed in the environment.

Définition de l'unité

One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to dichlorodimedon per min at pH 2.75 at 25 °C in the presence of potassium chloride and H₂O₂.

Forme physique

Crude suspension in 0.1 M sodium phosphate, pH ~4.5

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)