C3260

Citrate Synthase from porcine heart

ammonium sulfate suspension, ≥100 units/mg protein

• Synonyme(s) : Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)
• CAS Number: 9027-96-7
• Numéro CE : 4.1.3.7 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130808
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Porcine heart
• Niveau de qualité: 200
• Forme: ammonium sulfate suspension
• Activité spécifique: ≥100 units/mg protein
• Poids mol.: 98 kDa ( 49 kDa monomer)
• Solubilité: H₂O: soluble 1.0 mg/mL, clear
• Activité étrangère: isocitrate dehydrogenase and aconitase ≤0.01%; malic dehydrogenase ≤0.1%
• Température de stockage: 2-8°C

Description

Application

Citrate Synthase from porcine heart has been used:

• to inject newt egg for determining its importance in egg activation at fertilization
• in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
• to examine whether it can induce a [Ca²⁺] increase and egg activation in unfertilized eggs

Actions biochimiques/physiologiques

Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H₂O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.

Définition de l'unité

One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

Forme physique

Suspension in 3.2 M (NH₄)₂SO₄ solution, pH 7.0.

Notes préparatoires

Dissolves in water to form a clear solution at 1 mg/mL concentration.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable