C1057

Butyrylcholinesterase from equine serum

lyophilized powder, ≥900 units/mg protein

• Synonyme(s) : Acylcholine acyl-hydrolase, Choline esterase, butyryl, Pseudocholinesterase
• CAS Number: 9001-08-5
• Numéro CE : 3.1.1.8 (BRENDA, IUBMB)
• Numéro CE : 232-579-2
• Numéro MDL: MFCD00081455
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: lyophilized powder
• Activité spécifique: ≥900 units/mg protein
• Poids mol.: tetramer 440 kDa
• Composition: Protein, ≥10%
• Solubilité: H₂O: soluble 10 mg/mL
• Application(s): diagnostic assay manufacturing
• Température de stockage: −20°C

Description

Application

Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity has been explored. It has been reported that the level of BChE in human blood correlates to the degree of protection against potentially toxic nerve agents. Cholinesterases have also been investigated for their role in Alzheimer′s disease.

Actions biochimiques/physiologiques

Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities.BChE can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). The enzyme is activated by Ca²⁺ and Mg²⁺. The activity is constant over the pH range of 6.0-8.0. It is inhibited by betaine, nicotine, organophosphates, and carbamate.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.

Forme physique

Highly purified; contains buffer salts

Remarque sur l'analyse

Protein determined by biuret

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)